沙蚕胞外血红蛋白的亚基。

Subunits of the extracellular hemoglobin of Arenicola marina.

作者信息

Vinogradov S N, Kosinski T F, Kapp O H

出版信息

Biochim Biophys Acta. 1980 Feb 27;621(2):315-23. doi: 10.1016/0005-2795(80)90183-x.

Abstract

The molecular weight of the extracellular hemoglobin of Arenicola marina determined by equilibrium sedimentation is 3.74 +/- 0.12 . 10(6). Its iron content is 0.244 +/- 0.005 wt.% corresponding to a minimum molcular weight of 22 900 +/- 500. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate showed that the hemoglobin dissociated into three subunits: 14 000 (subunit 1), 31 000 (subunit 2) and 49 000 (subunit 3); in the presence of 2-mercaptoethanol four subunits were observed, 14 000 (subunit I), 16 000 (subunit II), 31 000 (subunit III), and 35 000 (subunit IV). Two-dimensional electrophoresis showed that subunit 1 produced subunit I, and subunits 2 and 3 produced subunits I, II and variable amounts of subunits III and IV. Gel filtration of reduced and alkylated A. marina hemoglobin in 6 M guanidinium hydrochloride suggests that the molecular weight of subunits I and II is 17 500 +/- 1000. A. marina hemoglobin appears to consist of at least 5--7 polypeptide chains. It is proposed that some of the polypeptide chains can associate to form dimers (subunits 2, III, IV) or trimers (subunit 3).

摘要

通过平衡沉降法测定的沙蠋细胞外血红蛋白的分子量为3.74±0.12×10⁶。其铁含量为0.244±0.005 wt.%，对应的最小分子量为22900±500。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，血红蛋白解离为三个亚基：14000（亚基1）、31000（亚基2）和49000（亚基3）；在2-巯基乙醇存在下观察到四个亚基，14000（亚基I）、16000（亚基II）、31000（亚基III）和35000（亚基IV）。二维电泳显示亚基1产生亚基I，亚基2和3产生亚基I、II以及不同量的亚基III和IV。在6M盐酸胍中对还原和烷基化的沙蠋血红蛋白进行凝胶过滤表明，亚基I和II的分子量为17500±1000。沙蠋血红蛋白似乎由至少5 - 7条多肽链组成。有人提出，一些多肽链可以缔合形成二聚体（亚基2、III、IV）或三聚体（亚基3）。