Structural investigation of the capsular polysaccharide of Klebsiella serotype K44.

The capsular polysaccharide from Klebsiella K44 has been investigated by the techniques of methylation, base-catalyzed elimination, Smith degradation, and partial hydrolysis. The last-named yielded an oligosaccharide corresponding to one repeating unit. The anomeric configurations of the sugar residues were determined by 1H- and 13C-n.m.r. spectroscopy. The polysaccharide has a fractional acetyl content and is the first in this series to be based on a linear, pentasaccharide repeating unit. leads to 3)-beta-D-Glcp-(1 leads to 4)-alpha-D-Glcp-(1 leads to 4)-beta-D-GlcpA-(1 leads to 2)-alpha-L-Rhap-(1 leads to 3)-alpha-L-Rhap-(1 leads to.