Helms R A, Bull D M
Gastroenterology. 1980 Apr;78(4):738-44.
A human colon cancer cell line, HCT-8, is shown to be an appropriate target cell for study of natural killer (NK) activity in man. In parallel experiments, effector cell characteristics for NK and antibody-dependent cellular cytotoxicity (ADCC) were found to be vested in a single lymphocyte subpopulation, which bore receptors for the Fc fragment of IgG, but lacked other surface receptors. The effector cell failed to adhere to glass and was inactivated by exposure to 3500 rad x-irradiation. Cells forming rosettes with SRBC and cells bearing receptors for complement were inactive in both systems. A wide distribution of NK activity was noted among individuals that correlated with the distribution of effector cell activity in ADCC (r = 0.8). Preincubation of NK effector cells with antibody-coated ADCC target cells markedly reduced NK activity. Neuraminidase treatment of effector cells led to increased NK and diminished ADCC, while trypsin treatment led to reduced NK activity and showed no effect on ADCC. Thus, NK and ADCC effector cells are highly overlapping if not identical populations, but different structures on the cell membrane may mediate the two activities.
一种人结肠癌细胞系HCT - 8被证明是研究人类自然杀伤（NK）活性的合适靶细胞。在平行实验中，发现NK和抗体依赖性细胞毒性（ADCC）的效应细胞特征存在于单个淋巴细胞亚群中，该亚群带有IgG Fc片段的受体，但缺乏其他表面受体。效应细胞不黏附于玻璃，经3500拉德X射线照射后失活。与SRBC形成花环的细胞和带有补体受体的细胞在这两种系统中均无活性。在个体中观察到NK活性分布广泛，且与ADCC中效应细胞活性的分布相关（r = 0.8）。用抗体包被的ADCC靶细胞预孵育NK效应细胞可显著降低NK活性。用神经氨酸酶处理效应细胞会导致NK活性增加而ADCC活性降低，而用胰蛋白酶处理则导致NK活性降低且对ADCC无影响。因此，NK和ADCC效应细胞即使不是完全相同的群体，也是高度重叠的，但细胞膜上不同的结构可能介导这两种活性。
