大鼠肝微粒体中维生素K依赖性羧化活性的分级分离与重组

Fractionation and reconstitution of vitamin K-dependent carboxylation activity in rat liver microsomes.

作者信息

Price J A, Bartels V L, Chiu A, Delaney R, Johnson B C

出版信息

J Biol Chem. 1980 Mar 10;255(5):1808-11.

Abstract

Attempts to purify the vitamin K-dependent carboxylation system from rat liver microsomes have not yet met with success. Purification procedures result in low yields of activity even after relatively mild separation methods. We have been able to demonstrate that one reason for these failures is that there are at least two components (which can be separated) necessary for the reaction. Recombination of the separated components is possible if the detergent (Triton X-100) concentration is lowered and ethylene glycol is added to the system. Under these conditions, vitamin K-dependent carboxylation of synthetic pentapeptide (Phe-Leu-Glu-Glu-Leu) can be totally reconstituted.

摘要

从大鼠肝脏微粒体中纯化维生素K依赖性羧化系统的尝试尚未成功。即使采用相对温和的分离方法，纯化程序得到的活性产率也很低。我们已经能够证明，这些失败的一个原因是该反应至少需要两种（可分离的）成分。如果降低去污剂（Triton X-100）浓度并向系统中添加乙二醇，则分离出的成分有可能重新组合。在这些条件下，合成五肽（苯丙氨酸-亮氨酸-谷氨酸-谷氨酸-亮氨酸）的维生素K依赖性羧化可以完全重建。