用离子选择性微电极测量非洲爪蟾胚胎中的游离钙。
Free calcium in Xenopus embryos measured with ion-selective microelectrodes.
作者信息
Rink T J, Tsien R Y, Warner A E
出版信息
Nature. 1980 Feb 14;283(5748):658-60. doi: 10.1038/283658a0.
A regulatory role for intracellular free calcium has been suggested in cell division and intercellular communication via gap junctions. Previous measurements of cytoplasmic free Ca2+ during cell division and uncoupling have been made with the Ca2+-sensitive photoprotein aequorin. We now report experiments using Ca2+-sensitive microelectrodes to monitor free Ca2+ in the cells and intercellular fluid of early embryos of the amphibian Xenopus laevis. In addition to measuring basal levels, we have looked for changes in free Ca2+ during cell division and during electrical uncoupling of the normally coupled embryonic cells, induced by acidification of the intracellular medium.
细胞内游离钙在细胞分裂以及通过间隙连接进行的细胞间通讯中具有调节作用，这一点已被提出。先前在细胞分裂和解偶联过程中对细胞质游离Ca2+的测量是使用对Ca2+敏感的光蛋白水母发光蛋白进行的。我们现在报告使用Ca2+敏感微电极监测非洲爪蟾早期胚胎细胞和细胞间液中游离Ca2+的实验。除了测量基础水平外，我们还研究了细胞分裂过程中以及细胞内介质酸化诱导的正常偶联胚胎细胞电偶联解除过程中游离Ca2+的变化。
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