Gross J, Staak R, Syllm-Rapoport I
Acta Biol Med Ger. 1978;37(3):403-8.
The activity of inorganic pyrophosphatase (PPase) of red blood cells (rbc) from newborns is about 40% higher than that of adults. To answer the question whether the higher activity was due to the enhanced reticulocyte count of the newborn's cell population, the rbc of both newborns and adults were separated into different density subpopulations by means of isopycnic density gradient centrifugation in dextran. To judge the quality of separation in the individual age populations of rbc, the activity of glucose-6-phosphate dehydrogenase (G-6-PD) and the reticulocyte count were determined. The activity fall of PPase during maturation and aging of rbc is stronger than that of G-6-PD. The density subpopulations of rbc from newborns and adults with equal reticulocyte counts show the same PPase activities yet different G-6-PD activities. Obviously, the higher PPase activity of a cell population of newborns is due to the higher proportion of reticulocytes. The inorganic PPase is well suited as a guiding criterion to judge the maturity and age of rbc of newborns.
新生儿红细胞（rbc）的无机焦磷酸酶（PPase）活性比成年人高约40%。为了回答这种较高活性是否归因于新生儿细胞群体中网织红细胞计数增加的问题，通过在右旋糖酐中进行等密度密度梯度离心，将新生儿和成年人的红细胞分离成不同密度的亚群。为了判断红细胞各年龄群体的分离质量，测定了葡萄糖-6-磷酸脱氢酶（G-6-PD）的活性和网织红细胞计数。红细胞在成熟和衰老过程中PPase活性的下降比G-6-PD更强。网织红细胞计数相同的新生儿和成年人的红细胞密度亚群显示出相同的PPase活性，但G-6-PD活性不同。显然，新生儿细胞群体中较高的PPase活性是由于网织红细胞比例较高。无机PPase非常适合作为判断新生儿红细胞成熟度和年龄的指导标准。
