致癌嘌呤N-氧化物酯共价修饰多核苷酸中的所有常见碱基。

Carcinogenic purine N-oxide ester modifies covalently all common bases in polynucleotides.

作者信息

Mathews R A, Stöhrer G

出版信息

Chem Biol Interact. 1980 Jan;29(1):57-66. doi: 10.1016/0009-2797(80)90086-1.

Abstract

The carcinogen 1-methyl-3-hydroxyxanthine after esterification binds covalently to polynucleotides, RNA and DNA. All four ribopolynucleotides and poly(dT) are targets. Depending on reaction conditions, covalent binding is greatest to poly(A) followed by poly(U), poly(dT), poly(G), poly(C), RNA and DNA. Maximal covalent modification of DNA is one moiety per 360 nucleotides. All modified polynucleotides, RNA and DNA, except poly guanylic acid have been enzymatically digested and the major adducts characterized as nucleosides.

摘要

致癌物质1-甲基-3-羟基黄嘌呤酯化后与多核苷酸、RNA和DNA共价结合。所有四种核糖多核苷酸和聚（dT）都是靶点。根据反应条件，共价结合对聚（A）的亲和力最大，其次是聚（U）、聚（dT）、聚（G）、聚（C）、RNA和DNA。DNA的最大共价修饰是每360个核苷酸有一个部分。除聚鸟苷酸外，所有修饰的多核苷酸、RNA和DNA都已被酶消化，主要加合物被鉴定为核苷。