Rash J M, Jerkunica I, Sgoutas D S
Clin Chem. 1980 Jan;26(1):84-8.
To study lipid interference in steroid radioimmunoassays in which dextran-coated charcoal is used as the separating agent, we tested triolein and phosphatidylcholine as model hydrophobic and amphipathic lipids, respectively. Addition of either caused distortion of the standard curve to an extent that was inversely related to the polarity of the steroid molecule. Both lipids form a dispersion that entraps steroid molecules. When we increased the charcoal concentration, the effect of phosphatidylcholine addition was eliminated for assays of both polar and nonpolar steroids. In contrast, the effect from triacylglycerol was not corrected, particularly in assays of nonpolar steroids. We also studied mixtures of lipids mimicking the mixture of lipids extracted from plasma of normolipemic and hyperlipemic indviduals. The degree of lipemia that can be tolerated differs from assay to assay, and primarily varies directly with the polarity of the steroid being assayed.
为研究在以葡聚糖包被活性炭作为分离剂的类固醇放射免疫分析中脂质的干扰作用，我们分别测试了三油酸甘油酯和磷脂酰胆碱作为疏水性和亲两性脂质的模型。添加任何一种都会导致标准曲线变形，其程度与类固醇分子的极性呈负相关。两种脂质都会形成截留类固醇分子的分散体。当我们提高活性炭浓度时，对于极性和非极性类固醇的检测，添加磷脂酰胆碱的影响都被消除了。相比之下，三酰甘油的影响并未得到校正，尤其是在非极性类固醇的检测中。我们还研究了模拟从血脂正常和高脂血症个体血浆中提取的脂质混合物的脂质混合物。不同检测方法可耐受的脂血程度不同，并且主要与所检测类固醇的极性直接相关。
