Gottlieb A A, Maziarz G A, Tamaki N, Sutcliffe S B
J Immunol. 1980 Feb;124(2):885-92.
Fractions from human whole leukocyte lysates were prepared by sequential double dialysis against membranes with cut-offs of m.w. 12,000 and 3500, and by elution chromatography on Sephadex G-10. The effect of localized intracutaneous implantation of fractions with m.w. less than 3500 was studied. Two types of response were obtained: 1) the amplification of response to antigen to which the donor had preexisting immunity, and 2) the induction of inflammatory response histologically resembling delayed hypersensitivity in the absence of added antigen. The substances mediating these responses could be separated into unique components by use of a long (1 x 150 cm) G-10 column, or by hydroxylapatite chromatography. The active principles were derived from leukocyte extracts (as demonstrated by their absence from autologous RBC extracts prepared by identical methodology), and the histopathologic appearances after intracutaneous implantation were compatible with those of a delayed-hypersensitivity response. No evidence of transfer of antigen-specific information from a sensitive donor to a nonsensitive recipient was obtained under the conditions of study.
通过依次对截留分子量为12,000和3500的透析膜进行两次透析，以及在葡聚糖凝胶G - 10上进行洗脱色谱，制备人全白细胞裂解物的组分。研究了皮下局部植入分子量小于3500的组分的效果。获得了两种类型的反应：1）对供体预先存在免疫的抗原的反应增强，以及2）在不添加抗原的情况下，组织学上类似于迟发型超敏反应的炎症反应的诱导。通过使用长（1×150 cm）的G - 10柱或羟基磷灰石色谱，可以将介导这些反应的物质分离成独特的组分。活性成分源自白细胞提取物（通过相同方法制备的自体红细胞提取物中不存在这些成分证明），皮下植入后的组织病理学表现与迟发型超敏反应的表现相符。在研究条件下，未获得抗原特异性信息从敏感供体转移到非敏感受体的证据。
