Siefermann-Harms D, Michel J M, Collard F
Biochim Biophys Acta. 1980 Feb 8;589(2):313-23.
The blue absorbance change occurring in flashed bean (Phaseolus vulgaris L.) leaves when exposed to continuous light (first observed by Strasser; Strasser, R.J. (1973) Arch. Int. Physiol. Biochem. 81, 935--955) is caused by the conversion of the following xanthophylls: violaxanthine leads to antheraxanthine leads to zeaxanthine. This conclusion is derived from the simultaneous occurrence of both reactions: (a) In flashed leaves, blue absorbance change and xanthophyll conversion take place under strong (2 mW . cm-2) but not under weak (0.02 mW . cm-2) white light. (b) In chloroplasts isolated from flashed leaves, the blue absorbance change occurs in the dark under conditions that also induce the xanthophyll conversion. (c) Blue absorbance change and xanthophyll conversion are both inhibited by dithiothreitol. In addition, the light-induced blue absorbance change is reversed in the dark if aerobic conditions are maintained, i.e. under conditions that in normal leaves favor the reversal of the above reaction sequence. The significance of the xanthophyll conversion is discussed in relation to other phenomena occurring in flashed leaves after exposure to continuous illumination.
菜豆（Phaseolus vulgaris L.）叶片在受到连续光照时出现的蓝色吸光度变化（最初由施特拉塞尔观察到；施特拉塞尔，R.J.（1973年）《国际生理学与生物化学杂志》81卷，935 - 955页）是由以下叶黄素的转化引起的：紫黄质转化为环氧玉米黄质再转化为玉米黄质。这一结论源于以下两个反应的同时发生：（a）在闪光处理的叶片中，蓝色吸光度变化和叶黄素转化在强光（2毫瓦·平方厘米⁻²）下发生，而在弱光（0.02毫瓦·平方厘米⁻²）白光下不发生。（b）在从闪光处理的叶片中分离出的叶绿体中，蓝色吸光度变化在黑暗中且在能诱导叶黄素转化的条件下发生。（c）蓝色吸光度变化和叶黄素转化都受到二硫苏糖醇的抑制。此外，如果保持有氧条件，即在正常叶片中有利于上述反应序列逆转的条件下，光诱导的蓝色吸光度变化在黑暗中会逆转。本文结合闪光处理的叶片在连续光照后出现的其他现象，讨论了叶黄素转化的意义。
