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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Nozawa M, Tanizawa K, Kanaoka Y

Biochim Biophys Acta. 1980 Feb 14;611(2):314-22. doi: 10.1016/0005-2744(80)90067-4.

'Inverse'-type substrates for butyrylcholinesterase (acylcholine acylhydrolase, EC 3.1.1.8), i.e., p- and o-nitrophenyl esters of (3-carboxypropyl)-trimethylammonium iodide, (4-carboxybutyl)trimethylammonium iodide and (5-carboxypentyl)trimethylammonium iodide were prepared, and their kinetic parameters for butyrylcholinesterase-catalyzed hydrolysis were determined. 2. The hydrolysis of these 'inverse'-type substrates were found to proceed through specific binding with the enzyme and efficient production of acyl enzyme intermediates, a pathway essentially identical with that followed by choline esters, normal type substrates.

制备了丁酰胆碱酯酶（酰基胆碱酰基水解酶，EC 3.1.1.8）的“反向”型底物，即（3-羧丙基）-三甲基碘化铵、（4-羧丁基）三甲基碘化铵和（5-羧戊基）三甲基碘化铵的对硝基苯酯和邻硝基苯酯，并测定了它们在丁酰胆碱酯酶催化水解反应中的动力学参数。2. 发现这些“反向”型底物的水解是通过与酶的特异性结合和酰基酶中间体的高效生成来进行的，这一途径与胆碱酯（正常型底物）所遵循的途径基本相同。

Nozawa M, Tanizawa K, Kanaoka Y

Biochim Biophys Acta. 1980 Feb 14;611(2):314-22. doi: 10.1016/0005-2744(80)90067-4.

'Inverse'-type substrates for butyrylcholinesterase (acylcholine acylhydrolase, EC 3.1.1.8), i.e., p- and o-nitrophenyl esters of (3-carboxypropyl)-trimethylammonium iodide, (4-carboxybutyl)trimethylammonium iodide and (5-carboxypentyl)trimethylammonium iodide were prepared, and their kinetic parameters for butyrylcholinesterase-catalyzed hydrolysis were determined. 2. The hydrolysis of these 'inverse'-type substrates were found to proceed through specific binding with the enzyme and efficient production of acyl enzyme intermediates, a pathway essentially identical with that followed by choline esters, normal type substrates.

制备了丁酰胆碱酯酶（酰基胆碱酰基水解酶，EC 3.1.1.8）的“反向”型底物，即（3-羧丙基）-三甲基碘化铵、（4-羧丁基）三甲基碘化铵和（5-羧戊基）三甲基碘化铵的对硝基苯酯和邻硝基苯酯，并测定了它们在丁酰胆碱酯酶催化水解反应中的动力学参数。2. 发现这些“反向”型底物的水解是通过与酶的特异性结合和酰基酶中间体的高效生成来进行的，这一途径与胆碱酯（正常型底物）所遵循的途径基本相同。