Rider D M, McDonagh R P, McDonagh J
Blood. 1980 Apr;55(4):559-63.
A method for radiolabeling platelets in vivo was developed in which 3H-arginine was injected into the bone marrow of normal dogs. On the third day after injection, a maximum of 6%--7% of the radioactivity had been incorporated into the total platelet mass. This method of isotope administration resulted in a 50--60-fold increase in maximum uptake of radiolabel by platelets, as compared to values obtained by others using intravenous injections of various radioactive compounds. Tritium-labeled platelets were harvested from the animals and then were washed to remove unbound 3H-arginine. On polyacrylamide gel electrophoresis 7 labeled protein bands, with molecular weights ranging from 29,000to 132,000, were obtained from the platelet-soluble fraction. One 3H-containing protein with a molecular weight of 81,000 was identified immunologically and enzymatically as platelet factor XIII.
已开发出一种在体内对血小板进行放射性标记的方法，即向正常犬的骨髓中注射³H-精氨酸。注射后第三天，放射性物质最多有6% - 7%被整合到血小板总量中。与其他人通过静脉注射各种放射性化合物所获得的值相比，这种同位素给药方法使血小板对放射性标记的最大摄取量增加了50 - 60倍。从动物体内采集氚标记的血小板，然后进行洗涤以去除未结合的³H-精氨酸。在聚丙烯酰胺凝胶电泳中，从血小板可溶性部分获得了7条分子量在29,000至132,000之间的标记蛋白带。一种分子量为81,000的含³H蛋白经免疫学和酶学鉴定为血小板因子XIII。
