Hernández-Yago J, Knecht E, Martínez-Ramón A, Grisolía S
Cell Tissue Res. 1980;205(2):303-9. doi: 10.1007/BF00234688.
Ferritin was trapped in negatively charged liposomes consisting of phosphatidyl choline and phosphatidyl serine in a molar ratio of 9:1. Unilamellar vesicles were prepared by ultrasonic treatment. HeLa cells were incubated with these liposomes for 2h and chased for 0, 2, 14 and 20 h. The migration pathway of ferritin in the cells was followed by quantitative electron microscopy. Ferritin was detected exclusively in cytosol and lysosomes. Evidence is presented that free ferritin in cytosol enters lysosomes.
铁蛋白被包裹在由摩尔比为9:1的磷脂酰胆碱和磷脂酰丝氨酸组成的带负电荷的脂质体中。通过超声处理制备单层囊泡。将HeLa细胞与这些脂质体孵育2小时，然后分别在0、2、14和20小时进行追踪观察。通过定量电子显微镜追踪细胞中铁蛋白的迁移途径。铁蛋白仅在细胞质溶胶和溶酶体中被检测到。有证据表明细胞质溶胶中的游离铁蛋白进入了溶酶体。
