Panara F, Cirotto C, Arangi I, Barberini L
Experientia. 1980 Jan 15;36(1):119-20. doi: 10.1007/BF02004010.
3 molecular forms (P1, P2 and P3) of acid phosphatase (E.C. 3.1.3.2) have been detected in chicken liver homogenate. The different intracellular localization of these molecules has been demonstrated by cellular fractionation and electrophoretic analysis. P1 and P2 phosphatases are both present in the particulate fraction. P3 is present in a pure form in the soluble fraction. The difference between the enzyme molecules present in the particulate fraction and that in the soluble one is confirmed by the different activation-inhibition effect of various ions and substances on the enzymatic activity of subcellular fractions.
在鸡肝匀浆中已检测到酸性磷酸酶（E.C. 3.1.3.2）的3种分子形式（P1、P2和P3）。通过细胞分级分离和电泳分析证明了这些分子在细胞内的不同定位。P1和P2磷酸酶均存在于颗粒部分。P3以纯形式存在于可溶性部分。颗粒部分和可溶性部分中存在的酶分子之间的差异通过各种离子和物质对亚细胞部分酶活性的不同激活-抑制作用得到证实。
