Matsuzaki S, Iwamura K, Katsunuma T, Kamiguchi H
Gastroenterol Jpn. 1980;15(1):33-40. doi: 10.1007/BF02773702.
A method for separation of serum (pseudo) cholinesterase isozymes was studied in order to take advantage of it for clinical research. By employing modifications on a previously reported method, analytical time was shortened to a half (1.5 days) of the original method without abolishing qualitative and quantitative accuracy. Thus, the present method facilitated its clinical application for the study of this isozyme. A normal pattern of the isozyme in Japanese in this method was determined by the analysis of sixteen normal subjects, which appeared to be very consistent in each individual under the present conditions. The distribution of relative activities of respective isozymes measured on a densitogram coincided well with that measured calorimetrically. Abnormalities of the zymogram were newly found in patients with liver cirrhosis and metastatic liver cancer, which seemed to be characteristic to the respective diseases. Isozyme patterns in the liver and ascites were also measured and compared with those in serum.
为了将血清（拟）胆碱酯酶同工酶的分离方法应用于临床研究，对其进行了研究。通过对先前报道方法的改进，分析时间缩短至原方法的一半（1.5天），同时不影响定性和定量准确性。因此，本方法便于其在该同工酶研究中的临床应用。通过对16名正常受试者的分析，确定了该方法中日本人同工酶的正常模式，在当前条件下，每个个体的模式似乎非常一致。在密度图上测量的各同工酶相对活性分布与比色法测量的结果非常吻合。在肝硬化和转移性肝癌患者中发现了新的酶谱异常，这似乎是各疾病的特征。还测量了肝脏和腹水中的同工酶模式，并与血清中的进行了比较。
