牛眼晶状体α-晶状体蛋白的光散射研究。

Light scattering studies on alpha-crystallin from bovine eye lens.

作者信息

Sano Y

出版信息

J Biochem. 1980 Jan;87(1):15-21. doi: 10.1093/oxfordjournals.jbchem.a132720.

DOI:10.1093/oxfordjournals.jbchem.a132720

Abstract

Using the usual angular light scattering technique, we have measured the concentration dependence of reduced scattering intensity of fractionated bovine lens alpha-crystallin (alpha A-chain) in the presence of 30 mM calcium chloride and 0.1 M sodium chloride. The inverse intensity of scattered light at zero angle per unit concentration of alpha-crystallin has a significant upward curvature with increasing alpha-crystallin concentration. The third virial coefficient determined from this upward curvature was 1.24 x 10(-3) (mol 1/2 ml 1/2 g-1).

摘要

采用常规的角光散射技术，我们测量了在含有30 mM氯化钙和0.1 M氯化钠的条件下，分级分离的牛晶状体α-晶状体蛋白（αA链）的比散射强度与浓度的关系。α-晶状体蛋白单位浓度下零角度散射光的反向强度随α-晶状体蛋白浓度增加呈现出显著的向上弯曲。由该向上弯曲确定的第三维里系数为1.24×10⁻³（mol¹/² ml¹/² g⁻¹）。