Kii R, Sakai K, Sano A, Yonezawa K, Hashimoto E, Yamamura H
J Biochem. 1980 Jan;87(1):267-71. doi: 10.1093/oxfordjournals.jbchem.a132733.
Guanyl-5'-yl imidodiphosphate (Gpp(NH)p), a nucleotide phosphohydrolase-resistant analog of GTP, caused inhibitory and stimulatory effects on the basal adenylate cyclase activity of rat synaptosomal fractions when manganese was present in the assay mixture, whereas the nucleotide caused only a stimulatory effect when magnesium was employed. In the presence of manganese, the inhibitory and stimulatory effects of Gpp(NH)p could be seen at around concentrations of 10(-7) M and 10(-4) M Gpp(NH)p, respectively. The inhibitory and stimulatory effects of Gpp(NH)p were both antagonized competitively by GTP; these effects of the analog were the opposite of those observed with GTP, which was stimulatory and inhibitory for fat call adenylate cyclase at 10(-7) M and 10(-4) M, respectively (Yamamura, H., Lad, P.M., and Rodbell, M. (1977) J. Biol. Chem. 252, 7964--7966). The degree of inhibition by Gpp(NH)p did not depend on the concentration of manganese nor on the addition of ethylene glycol bis(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid.
鸟苷-5'-亚氨二磷酸（Gpp(NH)p）是一种对核苷酸磷酸水解酶有抗性的GTP类似物，当测定混合物中存在锰时，它对大鼠突触体部分的基础腺苷酸环化酶活性产生抑制和刺激作用，而当使用镁时，该核苷酸仅产生刺激作用。在有锰存在的情况下，Gpp(NH)p的抑制和刺激作用分别在约10^(-7) M和10^(-4) M的Gpp(NH)p浓度下可见。Gpp(NH)p的抑制和刺激作用均被GTP竞争性拮抗；该类似物的这些作用与GTP所观察到的作用相反，GTP在10^(-7) M和10^(-4) M时分别对脂肪细胞腺苷酸环化酶具有刺激和抑制作用（山村，H.，拉德，P.M.，和罗德贝尔，M.（1977年）《生物化学杂志》252，7964 - 7966）。Gpp(NH)p的抑制程度不取决于锰的浓度，也不取决于乙二醇双（β-氨基乙醚）-N，N，N'，N'-四乙酸的添加。
