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线粒体外膜细胞色素b5样血红素蛋白：线粒体外膜细胞色素b。II。线粒体外膜细胞色素b对鱼藤酮不敏感的NADH-细胞色素c还原酶活性的贡献。

Cytochrome b5-like hemoprotein of outer mitochondrial membrane: OM cytochrome b. II. Contribution of OM cytochrome b to rotenone-insensitive NADH-cytochrome c reductase activity.

作者信息

Ito A

出版信息

J Biochem. 1980 Jan;87(1):73-80. doi: 10.1093/oxfordjournals.jbchem.a132754.

DOI:10.1093/oxfordjournals.jbchem.a132754
PMID:7358642
Abstract

The participation of OM cytochrome b in the rotenone-insensitive NADH-cytochrome c reductase activity of rat tissues was investigated in comparison with that of cytochrome b5, by using antibodies against these two cytochromes. The specificity of each antibody was confirmed by inhibition studies of NADH-cytochrome c reductase activities reconstituted from purified cytochromes and NADH-cytochrome b5 reductase. OM cytochrome b-mediated NADH-cytochrome c reductase activity was found in various tissues including liver, kidney, heart, and brain. The contribution of this activity to the total rotenone-insensitive NADH-cytochrome c reductase activity was high in heart and brain cells. NADH-cytochrome c reductase activity mediated by OM cytochrome b was principally localized in mitochondrial outer membrane. Immunoadsorption studies using antibody-coated polyacrylamide beads showed that significant OM cytochrome b-mediated activity is present in the microsomal membrane, and that cytochrome b5-mediated activity also exists in the mitochondrial outer membrane.

摘要

通过使用针对这两种细胞色素的抗体，研究了线粒体外膜细胞色素b与细胞色素b5相比，在大鼠组织对鱼藤酮不敏感的NADH-细胞色素c还原酶活性中的参与情况。通过对由纯化的细胞色素和NADH-细胞色素b5还原酶重构的NADH-细胞色素c还原酶活性的抑制研究，证实了每种抗体的特异性。在包括肝脏、肾脏、心脏和大脑在内的各种组织中发现了线粒体外膜细胞色素b介导的NADH-细胞色素c还原酶活性。这种活性在心脏和脑细胞中对总的鱼藤酮不敏感的NADH-细胞色素c还原酶活性的贡献很高。线粒体外膜细胞色素b介导的NADH-细胞色素c还原酶活性主要定位于线粒体外膜。使用抗体包被的聚丙烯酰胺珠进行的免疫吸附研究表明，微粒体膜中存在显著的线粒体外膜细胞色素b介导的活性，并且线粒体外膜中也存在细胞色素b5介导的活性。

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Cytochrome b5-like hemoprotein of outer mitochondrial membrane: OM cytochrome b. II. Contribution of OM cytochrome b to rotenone-insensitive NADH-cytochrome c reductase activity.线粒体外膜细胞色素b5样血红素蛋白：线粒体外膜细胞色素b。II。线粒体外膜细胞色素b对鱼藤酮不敏感的NADH-细胞色素c还原酶活性的贡献。
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Subcellular distribution of OM cytochrome b-mediated NADH-semidehydroascorbate reductase activity in rat liver.
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[Effect of thyroxine on the NADH cytochrome c reductase activity of microsomes and outer mitochondrial membrane of rat liver depending on age].[甲状腺素对不同年龄大鼠肝脏微粒体和线粒体外膜NADH细胞色素c还原酶活性的影响]
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Cytochrome b5-like hemoprotein/cytochrome b5 reductase complex in rat liver mitochondria has NADH-linked aquacobalamin reductase activity.大鼠肝脏线粒体中的细胞色素b5样血红蛋白/细胞色素b5还原酶复合物具有与NADH相关的水钴胺素还原酶活性。
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Participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in NADH-semidehydroascorbic acid reductase activity of rat liver.线粒体外膜细胞色素b5样血红蛋白（线粒体外膜细胞色素b）参与大鼠肝脏中NADH-半脱氢抗坏血酸还原酶的活性。
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NADH-cytochrome c reductase activity in cultured human lymphocytes. Similarity to the liver microsomal NADH-cytochrome b5 reductase and cytochrome b5 enzyme system.培养的人淋巴细胞中的NADH-细胞色素c还原酶活性。与肝微粒体NADH-细胞色素b5还原酶和细胞色素b5酶系统的相似性。
Arch Biochem Biophys. 1976 Sep;176(1):119-26. doi: 10.1016/0003-9861(76)90147-8.
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NADH-dependent aryl hydrocarbon hydroxylase in rat liver mitochondrial outer membrane.大鼠肝脏线粒体外膜中依赖NADH的芳烃羟化酶
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