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[脑脊液的诊断:半定量快速检测的应用。蛋白质在玻璃管和塑料管上吸附情况的研究(作者译)]

[Diagnosis of cerebrospinal fluid: the use of semiquantitative rapid tests. Investigation of the adsorbance of proteins to glass and plastic tubes (author's transl)].

作者信息

Kleine T O

出版信息

J Clin Chem Clin Biochem. 1980 Jan;18(1):7-11.

PMID:7359092
Abstract

The semiquantitative rapid tests used in the present study proved to be sufficiently sensitive for the estimation of erythrocytes in CSF samples, but they were inadequate with respect to the estimation of less than 100 microliter mononuclear and less than 10/microliter segmental neutrophilic leucocytes, of bilirubin, and albumin. Plastic tubes and cuvettes (e.g. of polystyrol) did not adsorb IgG, IgA, albumin or prealbumin from CSF samples, but adsorbed up to 80% of each purified single protein.

摘要

本研究中使用的半定量快速检测方法经证明对于脑脊液样本中红细胞的估算具有足够的敏感性,但对于估算少于100微升的单核细胞以及少于10/微升的分叶核嗜中性白细胞、胆红素和白蛋白而言,这些方法并不适用。塑料管和比色皿(如聚苯乙烯材质的)不会从脑脊液样本中吸附IgG、IgA、白蛋白或前白蛋白,但会吸附高达80%的每种纯化单一蛋白质。

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