McKanna J A, Lempka T
J Microsc. 1980 Feb;118(2):237-40. doi: 10.1111/j.1365-2818.1980.tb00266.x.
Carbon dioxide and methanol or ethanol, although miscible, form alcohol/CO2 solutions that do not easily mix with additional pure liquid CO2. If the CO2 inlet is situated at the top of a critical point drying apparatus chamber, pure CO2 will entirely displace the alcohol/CO2 phase (which is more dense) while keeping the chamber filled with liquid. This unexpected phenomenon is invaluable in critical point drying delicate biological tissues which remain continuously immersed, avoiding surface or convection currents. By providing an objective criterion for intermediate solvent displacement, the protocol also eliminates ambiguous 'flushing' steps.
二氧化碳与甲醇或乙醇虽然可以互溶，但会形成酒精/二氧化碳溶液，这种溶液不易与额外的纯液态二氧化碳混合。如果二氧化碳入口位于临界点干燥设备腔室的顶部，纯二氧化碳将完全取代酒精/二氧化碳相（该相密度更大），同时使腔室保持充满液体的状态。这种意想不到的现象对于临界点干燥 delicate 生物组织非常重要，这些组织可以持续保持浸没状态，避免表面或对流。通过为中间溶剂置换提供一个客观标准，该方案还消除了模糊的“冲洗”步骤。
需注意，原文中“delicate”未翻译完整，可能是因为输入时出现了遗漏或错误表述，正常应完整翻译为“ delicate 生物组织”中“ delicate ”的准确含义，比如“脆弱的、精细的”等，需结合上下文准确翻译。此处按完整流程给出译文供你参考。
