Romas N A, Veenema R J, Hsu K C, Tomashefsky P, Lattimer J K, Tannenbaum M
J Urol. 1980 Mar;123(3):392-5. doi: 10.1016/s0022-5347(17)55950-9.
Comparisons of the bone marrow and serum acid phosphatase values obtained by counter-immunelectrophoresis and the Roy biochemical test were made in 72 patients with and in 13 patients without prostatic cancer. The counter-immunoelectophoresis test, when positive at more than 1 international unit per liter, showed only 4.4 per cent falsely positive results. The Roy biochemical test, which used sodium thymolphthalein monophosphate as the substrate, had 65 per cent falsely positive bone marrow acid phosphatase levels. Conflicting reports regarding the value of bone marrow acid phosphatase determinations in patients with prostatic cancer result from the use of non-specific substrates in biochemical methods for measurement and from the trauma incidental to bone marrow aspiration, which releases many non-prostatic acid phosphatase enzymes. The use of immunoassay such as counter-immunoelectrophoresis minimizes this source of error.
对72例前列腺癌患者和13例非前列腺癌患者进行了骨髓和血清酸性磷酸酶值的比较，比较方法采用反向免疫电泳法和罗伊生化试验法。反向免疫电泳试验在每升超过1国际单位呈阳性时，假阳性结果仅为4.4%。以磷酸麝香草酚酞钠为底物的罗伊生化试验，其骨髓酸性磷酸酶水平假阳性率为65%。关于前列腺癌患者骨髓酸性磷酸酶测定值的报道相互矛盾，原因在于生化测量方法中使用了非特异性底物，以及骨髓穿刺带来的创伤，后者会释放许多非前列腺酸性磷酸酶。采用反向免疫电泳等免疫测定法可将这种误差来源降至最低。
