X射线照射人二倍体皮肤成纤维细胞后，次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）位点突变的表达时间及剂量-反应关系的测定
Determination of the expression time and the dose--response relationship for mutations at the HGPRT (hypoxanthine-guanine-phosphophoribosyl transferase) locus induced by X-irradiation in human diploid skin fibroblasts.
作者信息
de Ruijter Y C, Simons J W
出版信息
Mutat Res. 1980 Feb;69(2):325-32. doi: 10.1016/0027-5107(80)90097-4.
The length of the expression time for mutants resistant to 8-azaguanine or 6-thioguanine induced by X-rays was determined in human diploid skin fibroblasts. The cells were seeded in the selective medium over a period of 14 days after treatment. Direct expression of at least a part of the mutants was observed at day 0, and an increase of the mutant frequency over the entire cultivation period appeared to be due to spontaneous mutation. The dose-response relationship does not appear to deviate from linearity. The mutation rate per R had a mean value of 2.1 x 10(-7) which is about twice the value of the mutation rate found in rodent cells for the same locus.
在人二倍体皮肤成纤维细胞中测定了经X射线诱导的对8-氮杂鸟嘌呤或6-硫代鸟嘌呤耐药的突变体的表达时间长度。处理后14天内，将细胞接种于选择性培养基中。在第0天观察到至少一部分突变体的直接表达，并且在整个培养期间突变频率的增加似乎是由于自发突变。剂量反应关系似乎不偏离线性。每伦琴的突变率平均值为2.1×10⁻⁷，约为在啮齿动物细胞中同一基因座发现的突变率值的两倍。
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