Drewinko B, Yang L Y
Oncology. 1980;37(2):89-95. doi: 10.1159/000225412.
Some properties and kinetics of synthesis of the carcinoembryonic antigen (CEA-LoVo) produced by an established human colon carcinoma cell line were analyzed. CEA-LoVo was assayed by the method of Chu and Reynoso which was standardized against the activity of the First British Standard for CEA. CEA-LoVo was stable at -20 and 4 degrees C. At 37 degrees C, CEA-LoVo degraded at the rate of 1.4%/day in cell-free supernatants, and at the rate of 6.6%/day in the supernatants of monolayer cultures. CEA-LoVo was sensitive to enzymatic treatment ( approximately 55% loss) and extraction of PCA (greater than 70% loss). The elution profile of CEA-LoVo in concanavalin A-Sepharose B coincided with that of the First British Standard for CEA. No A or B blood group antigenic activity was noted. Studies employing immunofluorescent and horseradish peroxidase-labeled antibody techniques demonstrated heavy membrane and moderate intracytoplasmic localization. The greatest amount of net synthesis occurred for cells in stationary phase while CEA-LoVo release occurred maximally in lag phase.
分析了一种已建株的人结肠癌细胞系所产生的癌胚抗原（CEA-LoVo）的一些特性及合成动力学。CEA-LoVo采用Chu和Reynoso的方法进行测定，该方法以第一版CEA英国标准品的活性进行标准化。CEA-LoVo在-20℃和4℃时稳定。在37℃时，CEA-LoVo在无细胞上清液中以1.4%/天的速率降解，在单层培养物的上清液中以6.6%/天的速率降解。CEA-LoVo对酶处理敏感（约损失55%），对PCA提取敏感（损失大于70%）。CEA-LoVo在伴刀豆球蛋白A-琼脂糖B中的洗脱图谱与第一版CEA英国标准品的洗脱图谱一致。未检测到A或B血型抗原活性。采用免疫荧光和辣根过氧化物酶标记抗体技术的研究表明，其主要定位于细胞膜，中等程度定位于细胞质。静止期细胞的净合成量最大，而CEA-LoVo的释放量在延迟期最大。
