Edelfors S
Acta Pharmacol Toxicol (Copenh). 1980 Feb;46(2):133-7. doi: 10.1111/j.1600-0773.1980.tb02432.x.
Rats were treated with lithium added to the diet for five weeks (40 mmol LiCl/kg diet). The mean plasma lithium concentration was 0.48 mmol/l plasma, and the blood was drawn at 8 a.m. The brains were removed and synaptosomes were prepared according to the method of Gray & Whittaker (1962) and Bradford (1972). The synaptosomes were incubated for 120 min. with 32P-orthophosphate, either in a lithium-containing medium or in a lithium-free medium. The 32P-incorporation was lower in the synaptosomes from lithium-treated rats than the 32P-incorporation in synaptosomes from control rats regardless of the medium chosen. The results indicate that lithium treatment in vivo decreases the 32P-incorporation into synaptosomal phospholipids and that the effect remains after the removal of the lithium ion.
给大鼠喂食添加锂的饲料五周（40毫摩尔氯化锂/千克饲料）。上午8点采集血液，测得血浆锂平均浓度为0.48毫摩尔/升。取出大脑，按照Gray和Whittaker（1962年）以及Bradford（1972年）的方法制备突触体。将突触体在含锂培养基或无锂培养基中与32P-正磷酸盐一起孵育120分钟。无论选择何种培养基，锂处理大鼠的突触体中32P掺入量均低于对照大鼠突触体中的32P掺入量。结果表明，体内锂处理会降低32P掺入突触体磷脂的量，且去除锂离子后该效应仍然存在。
