Tomecko G W, Kleinberg M L, Latiolais C J, Prior R B, Pesko L J, Jones B C
Am J Hosp Pharm. 1980 Feb;37(2):211-5.
The effect on antibiotic stability of thawing, with microwave radiation, cefazolin sodium admixtures frozen in polyvinyl chloride minibags was studied. Two brands of cefazolin sodium (Ancef and Kefzol) were reconstituted and placed in 50-, 100- and 250-ml polyvinyl chloride minibags of 5% dextrose in water or 0.9% sodium chloride. The resulting solutions were assayed for antibiotic stability, using an agar disk diffusion technique, and for pH. The solutions were then stored at -20 degrees C for 48 hours, thawed to room temperature in a microwave oven, and kept at room temperature for four hours, after which they were reassayed for potency and pH. The results indicated that after the freeze-thaw process, the cefazolin sodium minibag admixtures retained at least 90% of their initial antimicrobial activity. The minimal pH changes could not be related to changes in antimicrobial activity, and no color changes could be detected visually. Using a microwave oven can greatly reduce thawing time of antibiotic admixtures. To maintain solution stability and prevent accidents, it is important to calibrate the oven, avoid solution overheating, and observe full precautions in oven operation.
研究了用微波辐射解冻聚氯乙烯小袋中冷冻的头孢唑林钠混合液对抗生素稳定性的影响。将两种品牌的头孢唑林钠（安赛福和凯福隆）复溶后，分别置于装有5%葡萄糖水溶液或0.9%氯化钠溶液的50毫升、100毫升和250毫升聚氯乙烯小袋中。采用琼脂纸片扩散技术测定所得溶液的抗生素稳定性及pH值。然后将溶液在-20℃下储存48小时，在微波炉中解冻至室温，并在室温下放置4小时，之后重新测定其效价和pH值。结果表明，经过冻融过程后，头孢唑林钠小袋混合液至少保留了其初始抗菌活性的90%。最小的pH值变化与抗菌活性的变化无关，且肉眼未检测到颜色变化。使用微波炉可大大缩短抗生素混合液的解冻时间。为保持溶液稳定性并防止事故发生，校准微波炉、避免溶液过热以及在微波炉操作中严格遵守预防措施非常重要。
