Daly J M, Reynolds H M, Rowlands B J, Dudrick S J, Copeland E M
Ann Surg. 1980 Mar;191(3):316-22. doi: 10.1097/00000658-198003000-00010.
The effects of nutritional manipulation on host body weight dynamics, tumor growth patterns and host-tumor responses to chemotherapy were studied in Sprague-Dawley rats with Walker-256 carcinosarcomas. Group I maintained throughout on a regular diet (RD) gained carcass weight steadily. Group II lost carcass weight while fed a protein-free diet (PFD) but rapidly gained weight after switching to RD on day 15. Mean tumor volume increased 105% in Group I from day 15 to 21, 218% in Group II and 77% in Group III (continued on PFD p less than 0.05). From day 21 to day 33 tumor growth patterns were similar in Groups I and II, while mean tumor volume eventually plateaued in Group III. In Study B, Group II animals were given Methotrexate (MTX-20 mg/kg) two days and six days after switching from PFD to RD. The mean change in tumor volume in the MTX-treated rats was 1.31 +/- 0.1 cm3 compared with 8.14 +/- 0.1 cm3 (p less than 0.001) in the saline-treated control rats. MTX did not significantly affect tumor growth patterns in Group III (PFD) rats. In Study A, protein-calorie malnutrition resulted in host carcass weight loss and tumor growth retardation while nutritional repletion restored host carcass weight and stimulated tumor growth. In Study B, MTX was maximally effective in tumor-bearing rats that were switched from PFD to RD demonstrating that nutritional manipulation can improve host nutritional status and increase tumor response to chemotherapy.
在患有Walker-256癌肉瘤的Sprague-Dawley大鼠中，研究了营养调控对宿主体重动态变化、肿瘤生长模式以及宿主-肿瘤对化疗反应的影响。第一组大鼠自始至终维持常规饮食（RD），其胴体重量稳步增加。第二组大鼠在喂食无蛋白饮食（PFD）期间胴体重量减轻，但在第15天改回RD后体重迅速增加。从第15天到21天，第一组大鼠的平均肿瘤体积增加了105%，第二组增加了218%，第三组增加了77%（继续喂食PFD，p<0.05）。从第21天到第33天，第一组和第二组大鼠的肿瘤生长模式相似，而第三组大鼠的平均肿瘤体积最终趋于平稳。在研究B中，第二组动物在从PFD改为RD后的第二天和第六天给予甲氨蝶呤（MTX-20mg/kg）。MTX处理大鼠的肿瘤体积平均变化为1.31±0.1cm³，而生理盐水处理的对照大鼠为8.14±0.1cm³（p<0.001）。MTX对第三组（PFD）大鼠的肿瘤生长模式没有显著影响。在研究A中，蛋白质-热量营养不良导致宿主胴体重量减轻和肿瘤生长迟缓，而营养补充恢复了宿主胴体重量并刺激了肿瘤生长。在研究B中，MTX对从PFD改为RD的荷瘤大鼠效果最佳，表明营养调控可以改善宿主营养状况并增强肿瘤对化疗的反应。
