Maruyama T
Biochim Biophys Acta. 1980 Mar 13;596(3):476-80. doi: 10.1016/0005-2736(80)90138-8.
The transepithelial shunt pathway of newt proximal tubule was examined with glass micro-electrode and electron microscopic methods. The input resistance of the peritubular (basal) membrane and tubular wall were found to be 4.2 +/- 0.1-10(6) (mean +/- S.E., n = 16) and 11.4 +/-0.2-10(4) (n = 11), respectively. The input resistance of the peritubular membrane was approximately 40-times larger than that of the tubular wall. When the kidneys were perfused in a lanthanum solution, the lanthanum ions were then observed in the junctional complexes and in the intercellular spaces on both the basal and apical sides. The results indicate that the electrical shunt pathway corresponds to the apical junctional complexes and the intercellular spaces, and that the tight junctions are not truly 'tight' for the transepithelial movement of small ions in the proximal tubule of the newt kidney.
采用玻璃微电极和电子显微镜方法研究了蝾螈近端小管的跨上皮分流途径。发现肾小管周围（基底）膜和管壁的输入电阻分别为4.2±0.1×10⁶（平均值±标准误，n = 16）和11.4±0.2×10⁴（n = 11）。肾小管周围膜的输入电阻约为管壁输入电阻的40倍。当肾脏在镧溶液中灌注时，随后在基底侧和顶端侧的连接复合体和细胞间隙中观察到镧离子。结果表明，电分流途径对应于顶端连接复合体和细胞间隙，并且紧密连接对于蝾螈肾脏近端小管中小离子的跨上皮移动并非真正“紧密”。
