Southern L L, Clawson A J
J Anim Sci. 1980 Mar;50(3):459-66. doi: 10.2527/jas1980.503459x.
Corn naturally contaminated with 2,000 ppb total aflatoxin was adjusted to 17% moisture and ammoniated (Am) with 1.5% (w/w) anhydrous ammonia in an attempt at detoxification. The ammonia treatment reduced the detectable concentration to 510 ppb total aflatoxins. Ninety-six male Sprague-Dawley rats (75 g avg) were randomly divided into eight treatment groups with 12 rats per treatment. A fortified 12%-protein corn-soybean meal diet served as the control (C) diet. The corn used in the C diet contained no detectable aflatoxin. Treatment 2 contained 1,670 ppb aflatoxin; treatments 3 and 4 were similar to treatments 1 and 2, respectively, except the corn used was ammoniated. Treatments 5 through 8 contained 830, 420, 210 and 100 ppb total aflatoxin, respectively. Liver weights expressed as a percentage of final live weight were increased (P less than .05) in rats consuming diets containing 1,670 ppb aflatoxin compared to the other treatments. Serum albumin, IgG and IgM concentrations were not affected by ammoniation or by concentration of aflatoxin in the corn. At the end of 4 weeks, total serum protein was increased (P less than .05) in rats consuming diets containing aflatoxin-contaminated (AfC) corn and Am corn compared to those consuming the C diet. Average daily gain and average daily feed consumption were reduced (P less than .01) in rats consuming the diet containing 1,670 ppb aflatoxin. The growth of rats consuming AfC corn that was Am was equal to that of rats consuming a similar concentration of aflatoxin by dilution. From these results and the results of others, it appears that ammoniation of corn contaminated with aflatoxin may be a practical and economical method for detoxification.
将天然受到黄曲霉毒素总量达2000 ppb污染的玉米调至17%的水分含量，并用1.5%（w/w）的无水氨进行氨化处理（Am），以尝试进行解毒。氨处理使可检测到的黄曲霉毒素总量浓度降至510 ppb。96只雄性斯普拉格-道利大鼠（平均体重75 g）被随机分为8个处理组，每组12只大鼠。一种强化的12%蛋白质玉米-豆粕日粮用作对照（C）日粮。C日粮中使用的玉米未检测到黄曲霉毒素。处理2含有1670 ppb黄曲霉毒素；处理3和4分别与处理1和2相似，只是所用玉米经过了氨化处理。处理5至8的黄曲霉毒素总量分别为830、420、210和100 ppb。与其他处理相比，食用含1670 ppb黄曲霉毒素日粮的大鼠肝脏重量占最终活体重量的百分比增加（P<0.05）。血清白蛋白、IgG和IgM浓度不受氨化处理或玉米中黄曲霉毒素浓度的影响。在4周结束时，与食用C日粮的大鼠相比，食用含黄曲霉毒素污染（AfC）玉米和氨化玉米日粮的大鼠血清总蛋白增加（P<0.05）。食用含1670 ppb黄曲霉毒素日粮的大鼠平均日增重和平均日采食量降低（P<0.01）。食用经氨化处理的AfC玉米的大鼠生长情况与通过稀释食用相似浓度黄曲霉毒素的大鼠相同。从这些结果以及其他研究结果来看，对受黄曲霉毒素污染的玉米进行氨化处理可能是一种实用且经济的解毒方法。
