Shimada K, Nagase Y
J Chromatogr. 1980 Jan 11;181(1):51-7. doi: 10.1016/s0378-4347(00)81268-2.
A quantitative high-performance liquid chromatographic method, using a polystyrene-divinyl benzene (Hitachi No. 3010 gel) column and aqueous methanol as the mobile phase, was employed for the determination of aminopyrine and its related compounds, 4-acetyl-aminoantipyrine, 4-aminoantipyrine and 4-monomethylaminoantipyrine. Baseline separation could be achieved within 25 min. The method was applied to the recovery of these materials from control urine and human urine. Before separation human urine was adjusted to pH 9 and extracted with ethyl acetate, chloroform and diethyl ether.
采用一种高效液相色谱定量方法，使用聚苯乙烯 - 二乙烯基苯（日立3010凝胶）柱，以甲醇水溶液作为流动相，用于测定氨基比林及其相关化合物4 - 乙酰氨基安替比林、4 - 氨基安替比林和4 - 单甲基氨基安替比林。25分钟内可实现基线分离。该方法应用于从对照尿液和人尿液中回收这些物质。分离前，将人尿液调节至pH 9，并用乙酸乙酯、氯仿和乙醚萃取。
