High-performance liquid chromatographic separation of carbamazepine metabolites excreted in rat urine.

A procedure for the separation and isolation of the urinary metabolites of carbamazepine by reversed-phase high-performance liquid chromatography is described. After extraction from urine, the metabolites were separated on either an analytical or semi-preparative C18 mu Bondapak column by gradient elution with methanol-water-acetic acid. Following derivatization the metabolites isolated by the use of the semi-preparative column were analyzed by gas chromatography and gas chromatography-mass spectrometry.