Chelberg R D, Gunawan S, Treiman D M
Neurology Service, Veterans Administration West Los Angeles Medical Center, California.
Ther Drug Monit. 1988;10(2):188-93. doi: 10.1097/00007691-198802000-00013.
A rapid high-performance liquid chromatographic method is described for the simultaneous determination of carbamazepine and the 10,11-epoxide, 10,11-dihydroxy, and 2-hydroxy metabolites of carbamazepine. The chromatographic system involves the use of a 18C-microsorb, reversed-phase column with acetonitrile/water (28:72) as the mobile phase. Detection and quantitation are monitored by ultraviolet absorption at 212 nm. The compounds are extracted from 250 microliters of plasma or from 100 microliters urine with methyl-t-butyl ether and 0.1 M sodium hydroxide; 2-methylcarbamazepine is added as internal standard. If phenytoin and/or phenobarbital are present in plasma or urine samples, it is necessary to use 1.0 M sodium hydroxide. The limits of quantitation for carbamazepine and its metabolites are 10 ng/ml.
描述了一种快速高效液相色谱法，用于同时测定卡马西平及其10,11 - 环氧化物、10,11 - 二羟基和2 - 羟基代谢物。色谱系统使用18C - 微球反相柱，以乙腈/水（28:72）作为流动相。通过在212nm处的紫外吸收进行检测和定量。用甲基叔丁基醚和0.1M氢氧化钠从250微升血浆或100微升尿液中提取化合物；加入2 - 甲基卡马西平作为内标。如果血浆或尿液样品中存在苯妥英和/或苯巴比妥，则有必要使用1.0M氢氧化钠。卡马西平及其代谢物的定量限为10ng/ml。
