Uvelli D A, Lee M Y, Manning J M, Hlastala M P, Babb A L
J Lab Clin Med. 1980 May;95(5):748-58.
The kinetics of HbS carbamylation in whole blood have been investigated under conditions anticipated in extracorporeal treatment systems. The reaction was well represented by a bimolecular, irreversible, second-order mechanism, and the overall carbamylation rate was enhanced by increasing the temperature and decreasing the pH and PO2. An expression was developed to predict the carbamylation rate for a range of experimental conditions. The relative amount of beta chain carbamylation was increased for those conditions under which the overall carbamylation rate was lowered, i.e., lower temperature, higher PO2, and higher pH. Morphological examination of cells with predominantly beta chain carbamylation showed that the antisickling effect, as measured by this technique, could be accounted for entirely by an increase in the oxygen affinity. Although this observation does not exclude an effect independent of change in the oxygen affinity of carbamylated hemoglobin, such an effect, if it occurs, is not detectable by this method. The results of this study were used to design a reaction vessel for an extracorporeal treatment system for sickle cell anemia patients.
在体外治疗系统预期的条件下，对全血中血红蛋白S（HbS）氨甲酰化的动力学进行了研究。该反应很好地符合双分子、不可逆的二级反应机制，通过升高温度、降低pH值和氧分压（PO2）可提高整体氨甲酰化速率。已开发出一个表达式来预测一系列实验条件下的氨甲酰化速率。在整体氨甲酰化速率降低的条件下，即较低温度、较高PO2和较高pH值时，β链氨甲酰化的相对量会增加。对主要为β链氨甲酰化的细胞进行形态学检查表明，通过该技术测量的抗镰变效应完全可以由氧亲和力的增加来解释。尽管这一观察结果并不排除氨甲酰化血红蛋白的氧亲和力变化之外的其他效应，但如果存在这种效应，用该方法是检测不到的。本研究结果被用于为镰状细胞贫血患者设计一种体外治疗系统的反应容器。
