Biologic and morphologic characteristics of urinary bladder neoplasms induced in BALB/c female mice with 2-acetylaminofluorene.

2-Acetylaminofluorene (2-AAF) induced transitional cell carcinomas, transitional cell carcinomas with squamous metaplasia, squamous cell carcinomas and undifferentiated carcinomas in the urinary bladders of BALB/c female mice. The transitional cell carcinoma was the most common, but was less aggressive and infiltrative than the other three types. The carcinomas were most likely to occur in the vertex of the bladder. A positive correlation existed between dose and length of administration of 2-AAF and the incidence, type, depth of invasion, and the location of the bladder carcinoma.