Blomquist E, Westermark B, Pontén J
Mech Ageing Dev. 1980 Feb;12(2):173-82. doi: 10.1016/0047-6374(80)90093-7.
A minicloning technique was used to analyse quantitatively the fraction of cells incapable of division in mass populations of human glial cells at various passage levels. The percentage of non-dividers rose from 18 to 73% between passages 11 and 40 at which further subcultivation of the mass culture became impossible. The non-dividers were predominantly arrested in G1; only a minor fraction of G2 arrested cells was established. Although not conclusive, the data suggest that commitment to irreversible loss of division potential increases as a function of the number of completed cell cycles which a glial cell has completed.
一种微量克隆技术被用于定量分析在不同传代水平的人神经胶质细胞大量群体中不能分裂的细胞比例。在传代11至40代之间，不能分裂的细胞百分比从18%上升至73%，此时大量培养物无法进一步传代。不能分裂的细胞主要停滞在G1期；仅确定了一小部分停滞在G2期的细胞。尽管尚无定论，但数据表明，神经胶质细胞对不可逆分裂潜能丧失的倾向随着其完成的细胞周期数增加而增加。
