Collins V P, Willems J S, Alva-Willems J M, Thaw H H
Mech Ageing Dev. 1984 Jul;26(1):1-12. doi: 10.1016/0047-6374(84)90160-x.
It has been reported that cultures of normal cells in phase II contain a non-multiplying cell population, the size of which increases with passage number. In phase II cultures of normal human glial cells we have found two subpopulations of non-proliferating cells, one of which has a characteristic morphology, and differs from the actively dividing cells in a number of respects: (1) they are larger although of various sizes and are well spread over a very large substratum area: (2) they contain a great number of granules showing acid phosphatase activity, being heavy metal positive and displaying the characteristic natural fluorescence of lipofuscin pigment; and (3) they frequently contained a central somewhat irregular nucleus with various numbers of darkly staining nucleolar-like structures. Cytophotometric nuclear DNA measurements of the described "large" cell population show a decreased proportion of diploid cells as compared to their smaller sister cells. Moreover, with increasing passage number, the DNA values for large cells shift towards higher ploidy levels resulting in a scattered aneuploid pattern in the oldest passage. This "large" cell subpopulation consists of between 2% and 3% of all passages and becomes greatly decreased following subcultivation. The other subpopulation of non-dividing cells is generally morphologically similar to the dividers, increases in size with passage number and is the more important in the phase III phenomenon.
据报道，处于II期的正常细胞培养物中含有一个非增殖细胞群体，其大小随传代次数增加。在正常人神经胶质细胞的II期培养物中，我们发现了两个非增殖细胞亚群，其中一个具有特征性形态，在许多方面与活跃分裂的细胞不同：（1）它们虽然大小各异，但更大，并且在非常大的底物区域上分布良好；（2）它们含有大量显示酸性磷酸酶活性的颗粒，呈重金属阳性，并显示脂褐素色素的特征性自发荧光；（3）它们经常含有一个中央有点不规则的细胞核，带有不同数量的深色核仁样结构。对所述“大”细胞群体进行细胞光度法核DNA测量显示，与较小的姐妹细胞相比，二倍体细胞的比例降低。此外，随着传代次数的增加，大细胞的DNA值向更高的倍性水平转移，导致最老传代中的非整倍体模式分散。这个“大”细胞亚群占所有传代细胞的2%至3%，传代培养后大大减少。另一个非分裂细胞亚群在形态上通常与分裂细胞相似，大小随传代次数增加，并且在III期现象中更为重要。
