Lupetti M, Dolfi A
Transplantation. 1980;29(1):67-71. doi: 10.1097/00007890-198001000-00014.
It has been proposed that the anatomical connection between the bursa of Fabricius and the cloaca is the pathway for unknown intestinal factors which are necessary for the induction of normal bursal lymphopoiesis. It has also been suggested that normal lymphopoiesis occurs only if nerve and vascular connections are intact. Experiments were performed to test these hypotheses. To test the influence of the intestinal contents, the bursal stalk was cut on the 16th day of incubation or at hatching. In this way, contact between the bursa and the intestinal flow in embryos was avoided and bacterial contamination of the bursa at hatching was also avoided. No change in the bursal follicles was observed. To study the influence of the nervous system on bursal lymphopoiesis, the bursa was isolated from the cloaca at hatching, and by maintaining vascularization the bursa was sutured to the peritoneum of the abdominal wall after scratching the contact surfaces. Once a new vascular network was established, one fragment of bursa was completely isolated from its normal anatomical site, causing interruption of the blood vessels and nerves of the bursa. The histological appearance of the bursa was not changed. It would appear that the integrity of the anatomical relation between bursa and cloaca and an intact nerve supply is not necessary for normal lymphopoiesis to occur in the bursa of Fabricius. In contrast, sufficient vascularization appears to be essential.
有人提出，法氏囊与泄殖腔之间的解剖学联系是未知肠道因子的通路，这些因子对于诱导正常的法氏囊淋巴细胞生成是必需的。也有人认为，只有神经和血管连接完整时才会发生正常的淋巴细胞生成。进行了实验来检验这些假设。为了测试肠道内容物的影响，在孵化第16天或孵化时切断法氏囊柄。通过这种方式，避免了法氏囊与胚胎肠道液的接触，也避免了孵化时法氏囊的细菌污染。未观察到法氏囊滤泡有变化。为了研究神经系统对法氏囊淋巴细胞生成的影响，在孵化时将法氏囊与泄殖腔分离，并在刮擦接触表面后通过维持血管化将法氏囊缝合到腹壁的腹膜上。一旦建立了新的血管网络，将一部分法氏囊从其正常解剖部位完全分离，导致法氏囊的血管和神经中断。法氏囊的组织学外观没有改变。看来，法氏囊与泄殖腔之间解剖关系的完整性以及完整的神经供应对于法氏囊中正常的淋巴细胞生成并非必要。相比之下，充足的血管化似乎至关重要。
