Gill P D
Biochem Genet. 1978 Jun;16(5-6):531-40. doi: 10.1007/BF00484217.
An investigation into isoenzymic analysis using four different buffer/gel systems was carried out. Fourteen different isoenzyme systems were surveyed on each buffer/gel system. It is shown that comparable results are not obtained between different systems. Previous workers in Cepaea nemoralis have used different buffer/gel systems from one another. Alkaline phosphatase and acid phosphatase and certain lactate and malate dehydrogenases are shown to behave similarly in three different systems. It is tentatively suggested that these isoenzymes may be genetically identical. "Nothing dehydrogenase" activity is demonstrated in gels stained for other dehydrogenases. It is suggested that nothing dehydrogenase activity may be attributable to glutamate or malate dehydrogenase.
对使用四种不同缓冲液/凝胶系统的同工酶分析进行了研究。在每种缓冲液/凝胶系统上对14种不同的同工酶系统进行了检测。结果表明，不同系统之间未获得可比的结果。先前研究黄斑蜗牛的人员使用了彼此不同的缓冲液/凝胶系统。碱性磷酸酶、酸性磷酸酶以及某些乳酸脱氢酶和苹果酸脱氢酶在三种不同系统中的表现相似。初步认为这些同工酶可能在基因上是相同的。在为其他脱氢酶染色的凝胶中显示出“无脱氢酶”活性。有人提出，无脱氢酶活性可能归因于谷氨酸脱氢酶或苹果酸脱氢酶。
