从海胆精子中分离出重卫星脱氧核糖核酸。

Isolation of heavy satellite deoxyribonucleic acid from sea-urchin spermatozoa.

作者信息

Mishra N K

出版信息

Biochem J. 1978 Oct 1;175(1):15-9. doi: 10.1042/bj1750015.

DOI:10.1042/bj1750015

PMID:736891

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186035/

Abstract

Two different methods were used to isolate high-molecular-weight heavy satellite DNA from the spermatozoa of the sea-urchin Lytechinus variegratus. Purification of satellite rDNA (ribosomal DNA) by selective denaturation of the spermatozoal DNA followed by a separation of the native and denatured DNA in an aqueous mixture of poly(ethylene glycol)/dextran is affected by the molecular weight of the spermatozoal DNA. Enrichment of satellite rDNA by selective precipitation of main-band DNA by poly-L-lysine is cumbersome but more suitable.

摘要

采用两种不同方法从海胆（Lytechinus variegratus）精子中分离高分子量重卫星DNA。通过精子DNA的选择性变性，随后在聚乙二醇/葡聚糖的水性混合物中分离天然和变性DNA来纯化卫星rDNA（核糖体DNA），这受到精子DNA分子量的影响。通过聚-L-赖氨酸选择性沉淀主带DNA来富集卫星rDNA虽然麻烦但更合适。