Amerault T E, Rose J E, Martin W E, Roby T O
Am J Vet Res. 1980 Mar;41(3):435-8.
Effects of adding phenol to sera used for the card-agglutination test (CAT) and for the micro-complement-fixation test (CFT) for bovine anaplasmosis were studied. Sera were obtained from 14 recently infected cattle, 17 cattle vaccinated with a killed anaplasmosis vaccine, 5 cattle in the carrier phase of the disease, and 45 cattle of unknown anaplasmosis status. Aliquots of sera were tested with and without phenol (0.25% final concentration). Phenol adversely affected the CAT by causing false-negative results. The CAT reactions of nonphenolized sera from recently infected cattle were all positive 4 weeks after inoculation, whereas CAT reactions of phenolized sera were not all positive until 10 weeks after inoculation. Nine non-phenolized sera from vaccinated cattle that were CAT-positive were CAT-negative after being phenolized. Phenolized sera from carrier cattle and from cattle of unknown anaplasmosis status were less reactive on the CAT than were nonphenolized sera. Effects of phenol on the CFT were not so obvious. Although phenol had little effect on end-titer results, it eliminated most prozones that cause false-negative CFT results at the 1:5 dilution. Without phenol, 30 of 215 sera would have been CFT-negative at this dilution because of prozones; with phenol, 6 would have been CFT-negative.
研究了向用于牛无浆体病的卡片凝集试验（CAT）和微量补体结合试验（CFT）的血清中添加苯酚的效果。血清取自14头近期感染的牛、17头接种了灭活无浆体病疫苗的牛、5头处于疾病携带阶段的牛以及45头无浆体病状况不明的牛。对血清等分试样分别在添加和不添加苯酚（终浓度0.25%）的情况下进行检测。苯酚会导致假阴性结果，从而对CAT产生不利影响。近期感染牛的未添加苯酚血清在接种后4周时CAT反应均为阳性，而添加苯酚血清的CAT反应直到接种后10周才全部呈阳性。9份接种牛的CAT阳性未添加苯酚血清在添加苯酚后变为CAT阴性。携带牛和无浆体病状况不明牛的添加苯酚血清在CAT上的反应性低于未添加苯酚的血清。苯酚对CFT的影响不那么明显。虽然苯酚对最终滴度结果影响不大，但它消除了大多数在1:5稀释度下导致CFT假阴性结果的前带现象。不添加苯酚时，215份血清中有30份因前带现象在该稀释度下CFT为阴性；添加苯酚后，只有6份CFT会为阴性。
