Barrow D A, Lentz B R
Biochim Biophys Acta. 1980 Mar 27;597(1):92-9. doi: 10.1016/0005-2736(80)90153-4.
Small, unilamellar phospholipid vesicles have been prepared using a new, high-powdered cup sonifier that avoids contact of the sample with a titanium probe. These vesicles have been characterized by gel filtration chromatography both before and after fractionation by high-speed centrifugation. Plots of the turbidity of centrifuged vesicles between 300 and 650 nm against the reciprocal fourth power of the scattering wavelength were linear with zero intercepts (extrapolated to infinite wavelength). In the presence of minute quantities of large, multilamellar vesicles, these plots remained linear but had intercepts quantitatively proportional to the amount of contaminating large vesicles. Since this measurement requires only a standard spectrophotometer and very small quantities of lipid, this method is suggested as a useful assay for determining contamination of small vesicle preparations by large vesicles. Two applications of this method as well as a practical limitation are discussed.
使用一种新型的高功率杯式超声破碎仪制备了小的单层磷脂囊泡，该超声破碎仪可避免样品与钛探头接触。在通过高速离心分级分离之前和之后，均通过凝胶过滤色谱法对这些囊泡进行了表征。将离心后的囊泡在300至650纳米之间的浊度相对于散射波长的倒数四次方作图，得到的曲线呈线性，截距为零（外推至无限波长）。在存在微量大的多层囊泡的情况下，这些曲线仍保持线性，但截距与污染的大囊泡数量成定量比例。由于该测量仅需要一台标准分光光度计和极少量的脂质，因此建议该方法作为一种有用的测定方法，用于确定小囊泡制剂中是否被大囊泡污染。讨论了该方法的两个应用以及一个实际限制。
