Roussel F, Laumonier R, Tayot J
Biomedicine. 1980 Feb;32(1):41-5.
1-2 dimethylhydrazine was injected subcutaneously on a weekly basis of 15 mg/kg, during periods ranging from one to twenty-eight weeks into five hundred Wistar rats. Glandular-mucin depletion appears as early as the second injection (sulfomucins disappear). From the sixth week, one observes dysplasia; if the animals are allowed to survive for a longer period all will develop colonic carcinomas, which were found mainly in the left colon. This dysplasia, which is first mild, then moderate, is finally severe in rat mucosa or in the area of polyps. Dysplastic polyps become less frequent when carcinomas increase in number and in size. The average length of survival after cessation of the exposure is inversely proportional to the total dose administered. The glandular mucin depletion-dysplasia-carcinoma sequence characterizes the types of formation of 1-2 DMH induced colonic carcinomas. One of the striking features is the early appearance of this sequence.
每周以15毫克/千克的剂量给500只Wistar大鼠皮下注射1,2 - 二甲基肼，持续1至28周。早在第二次注射时就出现腺黏液消耗（硫黏液消失）。从第六周开始，观察到发育异常；如果动物存活更长时间，所有动物都会发生结肠癌，主要发生在左结肠。这种发育异常最初较轻，然后中等，最终在大鼠黏膜或息肉区域变得严重。当癌的数量和大小增加时，发育异常性息肉变得不那么常见。接触停止后的平均存活时间与给予的总剂量成反比。腺黏液消耗 - 发育异常 - 癌序列是1,2 - 二甲基肼诱导的结肠癌形成类型的特征。其中一个显著特征是这个序列出现得很早。
