Lagerlöf F
Clin Chim Acta. 1980 Mar 28;102(2-3):127-35. doi: 10.1016/0009-8981(80)90025-x.
A new method for the determination of ionized calcium in parotid saliva utilizing an automatic ionized calcium analyzer (Orion Biomedical Model Space-Stat 20) was evaluated. The method showed good precision with a relative standard deviation of 2.9--3.8 per cent in the range 0.20--0.79 mmol . l-1 after 226 duplicate measurements. Mean recovery was 94.9% in the range of 0.6--1.8 mmol. l-1. Bovine mucin was found not to interfere with the calcium electrode. Differences in sodium concentration, ionic strength and pH between the standard solutions and samples caused systematic errors. These were corrected using experimentally derived formulas. The influence of pH on the concentration of ionized calcium was studied in 13 parotid saliva samples. A non-linear relationship ranging between 32 and 84% of the total calcium concentration was found.
评估了一种使用自动离子钙分析仪（奥立龙生物医学模型Space-Stat 20）测定腮腺唾液中离子钙的新方法。该方法在226次重复测量后，在0.20--0.79 mmol·l-1范围内显示出良好的精密度，相对标准偏差为2.9--3.8%。在0.6--1.8 mmol·l-1范围内平均回收率为94.9%。发现牛粘蛋白不干扰钙电极。标准溶液和样品之间的钠浓度、离子强度和pH值差异会导致系统误差。使用实验得出的公式对这些误差进行了校正。在13份腮腺唾液样本中研究了pH对离子钙浓度的影响。发现总钙浓度的32%至84%之间存在非线性关系。
