鸡胚细胞染色质组分中的DNA甲基化
DNA methylation in chromatin fractions of chick embryo cells.
作者信息
Remington A, Ranjekar P K
出版信息
Experientia. 1980 Feb 15;36(2):178-9. doi: 10.1007/BF01953717.
PMID:7371750
Abstract
Transcriptionally active chromatin was prepared from cultured fibroblasts of chick embryos by fractionation after partial digestion with DNAase II. The degree of DNA methylation in the active chromatin fraction is twice that of inactive or unfractionated chromatin in unsynchronized cells and 4 to 5 times greater at the beginning of the S-phase in synchronized cells.
摘要
通过用DNA酶II部分消化后分级分离，从鸡胚培养的成纤维细胞中制备转录活性染色质。活性染色质部分中的DNA甲基化程度是未同步化细胞中无活性或未分级染色质的两倍，在同步化细胞的S期开始时则高出4至5倍。
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