与萌发小麦胚多核糖体的聚腺苷酸序列结合的蛋白质。

Proteins bound to poly(A) sequences of polyribosomes from germinating wheat embryos.

作者信息

Iskhakov B K, Filimonov N G, Ajtkhozhin M A

出版信息

Biochim Biophys Acta. 1978 Dec 21;521(2):470-5. doi: 10.1016/0005-2787(78)90288-5.

Abstract

A preparation of purified polyribosomes was isolated from wheat embryos germinating for 1 h with [3H]adenosine. Treatment of polyribosomes with pancreatic ribonuclease leads to the liberation of particles containing poly(A) sequences. Poly(A)-containing complexes show a heterogeneous distribution in the region 8--15 S. The ability of 8--15 S material to be absorbed to membrane filters under the conditions of low ionic strength and its sensitivity to pronase indicate that poly(A) sequences in polyribosomes are associated with protein. Analysis of this protein by electrophoresis in a polyacrylamide gel demonstrates the presence of two polypeptides with the molecular weights equal to 86,000 and 52,000.

摘要

用[³H]腺苷处理萌发1小时的小麦胚，分离得到纯化的多核糖体制剂。用胰核糖核酸酶处理多核糖体可导致含有聚腺苷酸（poly(A)）序列的颗粒释放。含poly(A)的复合物在8 - 15 S区域呈现异质性分布。在低离子强度条件下，8 - 15 S物质被膜过滤器吸附的能力及其对链霉蛋白酶的敏感性表明，多核糖体中的poly(A)序列与蛋白质相关。通过聚丙烯酰胺凝胶电泳分析该蛋白质，结果显示存在两种分子量分别为86,000和52,000的多肽。