Aw T Y, Grigor M R
Biochim Biophys Acta. 1978 Dec 22;531(3):257-65. doi: 10.1016/0005-2760(78)90207-2.
The triacylglycerols from several natural sources have been used as substrates for in vitro assays of three different aspects of fat digestion and absorption. The triacylglycerol mixtures were chosen because they differed widely both in total fatty acid composition and in the intramolecular distribution of fatty acids. The assays were designed to test the rate of hydrolysis with pancreatic lipase, the ability to form insoluble calcium soaps during the hydrolysis, and the ability to form mixed micelles with bile during the hydrolysis. Differences in the rate of hydrolysis appeared to be related to the structure and the triacylglycerols from lard and human milk, both of which have palmitic acid esterified in the sn-2 position, were hydrolysed most rapidly. Similarly the ability to form calcium soaps was related to structure and those triacylglycerols which released saturated fatty acids on hydrolysis favoured calcium soap formation. While there was a range of abilities to form mixed micelles with bile no obvious correlation with structure was detected.
来自几种天然来源的三酰甘油已被用作体外脂肪消化和吸收三个不同方面检测的底物。选择这些三酰甘油混合物是因为它们在总脂肪酸组成和脂肪酸的分子内分布上都有很大差异。这些检测旨在测试胰脂肪酶的水解速率、水解过程中形成不溶性钙皂的能力以及水解过程中与胆汁形成混合微团的能力。水解速率的差异似乎与结构有关，猪油和人乳中的三酰甘油水解最快，二者在sn-2位都有棕榈酸酯化。同样，形成钙皂的能力也与结构有关，水解时释放饱和脂肪酸的那些三酰甘油有利于钙皂的形成。虽然与胆汁形成混合微团的能力存在一定范围，但未检测到与结构有明显相关性。
