Yang Y C, Hruby V J, Heward C B, Hadley M E
Int J Pept Protein Res. 1980 Feb;15(2):130-8.
The solid phase synthesis of alpha-melanocyte stimulating hormone (alpha-MSH) using the benzhydrylamine resin and a number of recently described side chain protecting groups is given. The carboxamide terminal peptide was obtained directly by treatment of the peptide resin with liquid hydrogen fluoride at 0 degrees in the presence of carbonium ion scavengers. The solid phase synthesis of the C-terminal carboxylate hormone, porcine beta-melanocyte stimulating hormone (beta p-MSH), using a 1% cross-linked Merrifield resin and the same side chain protecting groups as in the alpha-MSH synthesis also is presented. Purification of both peptides was carried out by conventional chromatographic techniques. Both hormones were fully active and beta p-MSH was slightly more potent than previously reported in the literature.
本文给出了使用二苯甲基胺树脂和一些最近描述的侧链保护基团固相合成α-黑素细胞刺激素（α-MSH）的方法。在碳正离子清除剂存在下，于0℃用液态氟化氢处理肽树脂，直接得到酰胺末端肽。本文还介绍了使用1%交联的 Merrifield树脂和与α-MSH合成中相同的侧链保护基团固相合成C末端羧酸盐激素——猪β-黑素细胞刺激素（β p-MSH）的方法。两种肽均通过常规色谱技术进行纯化。两种激素均具有完全活性，且β p-MSH的效力略高于文献中先前报道的效力。
