Clark W A, Salsbury R L
J Dairy Sci. 1980 Mar;63(3):375-8. doi: 10.3168/jds.S0022-0302(80)82942-0.
Mature dairy cows in late lactation were given capsules containing 26, 40, 60, or 80 g of D,L-methionine; 32, 49, 69, or 90 g of methionine hydroxy analog; or 1, 2, or 4 ml of dimethyl sulfide. Animals used as controls received capsules containing water. Milk was collected at hourly intervals for 5 h and analyzed for dimethyl sulfide content by gas chromatography. All experimental cows were managed and fed (mainly corn silage plus concentrate with some hay and pasture) with the rest of the herd but were limited to water and clover hay during the 5-h experimental periods. Administration of 60 or 80 g of D,L-methionine or 1, 2, or 4 ml of dimethyl sulfide resulted in the appearance of dimethyl sulfide in the milk. The concentration of dimethyl sulfide in milk from cows receiving orally administered dimethyl sulfide (2 or 4 ml) was greatest at 1 h. When D,L-methionine (60 or 80 g) was given, dimethyl sulfide concentrations were lower and peaked later in the sampling period. The maximum concentration of dimethyl sulfide in the milk was 4.5 ppb when 4 ml of dimethyl sulfide were administered orally. No dimethyl sulfide was in the milk for any amount of methionine hydroxy analog, for 26 or 40 g of D,L-methionine, or for control capsules.
对处于泌乳后期的成年奶牛投喂含有26克、40克、60克或80克D,L-蛋氨酸的胶囊;32克、49克、69克或90克蛋氨酸羟基类似物的胶囊;或1毫升、2毫升或4毫升二甲基硫醚的胶囊。用作对照的动物投喂含水的胶囊。每小时采集一次牛奶,持续5小时,并通过气相色谱法分析其中二甲基硫醚的含量。所有实验奶牛与牛群中的其他奶牛一起饲养和喂食(主要是玉米青贮饲料加精饲料以及一些干草和牧草),但在5小时的实验期间仅提供水和三叶草干草。投喂60克或80克D,L-蛋氨酸或1毫升、2毫升或4毫升二甲基硫醚后,牛奶中出现了二甲基硫醚。口服二甲基硫醚(2毫升或4毫升)的奶牛所产牛奶中,二甲基硫醚浓度在1小时时最高。投喂D,L-蛋氨酸(60克或80克)时,二甲基硫醚浓度较低,且在采样期后期达到峰值。口服4毫升二甲基硫醚时,牛奶中二甲基硫醚的最大浓度为4.5 ppb。对于任何量的蛋氨酸羟基类似物、26克或40克D,L-蛋氨酸或对照胶囊,牛奶中均未检测到二甲基硫醚。