Taylor R P, Waller S J, Haden C, Addis D J
J Immunol. 1980 Jun;124(6):2571-7.
The Farr assay and sucrose gradient ultracentrifugation were used to study the importance of kinetic factors on the size of antibody/DNA immune complexes prepared from SLE sera and dsDNA. We have found that significantly different numbers and sizes of antibody/DNA immune complexes can be formed by varying the time course of addition of a given amount of DNA to an SLE serum. For example, the introduction of additional DNA to a preincubated antibody-DNA system does not lead to reequilibration of that system with respect to the amount of DNA bound or the size of the complexes formed even after 1 hr at 37 degrees C. The potential implications of these observations with respect to the pathogenesis of SLE is discussed.
采用Farr检测法和蔗糖梯度超速离心法，研究动力学因素对由系统性红斑狼疮（SLE）血清和双链DNA（dsDNA）制备的抗体/DNA免疫复合物大小的影响。我们发现，通过改变向SLE血清中加入一定量DNA的时间进程，可形成数量和大小显著不同的抗体/DNA免疫复合物。例如，向预先孵育的抗体-DNA系统中加入额外的DNA，即使在37℃孵育1小时后，该系统在结合的DNA量或形成的复合物大小方面也不会重新达到平衡。本文讨论了这些观察结果对SLE发病机制的潜在影响。
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