Riley R L, Addis D J, Taylor R P
J Immunol. 1980 Jan;124(1):1-7.
The kinetics of dissociation of antibody-radiolabeled dsDNA (homogeneous PM2 DNA or sonicated dsDNA of m.w. 6 x 10(5)) complexes at 37 degrees C have been examined via three independent radioimmunoassays: the Farr, Millipore Filter, and PEG assays. Two different procedures were used to study the kinetics. Either excess unlabeled DNA or high salt concentrations were employed to induce complex dissociation. Our results suggest that typical SLE sera contain at least two distinct populations of anti-dsDNA antibodies. One population is of rather high avidity and dissociates slowly in the presence of excess DNA or high salt. The other population is of considerably lower avidity and is dissociated more rapidly under these conditions. The results of a double label dissociation kinetics study provide independent evidence supporting this hypothesis.
通过三种独立的放射免疫测定法（即法尔氏法、微孔滤膜法和聚乙二醇法）研究了抗体 - 放射性标记双链DNA（均一的PM2 DNA或分子量为6×10⁵的超声处理双链DNA）复合物在37℃下的解离动力学。采用了两种不同的程序来研究动力学。要么使用过量未标记的DNA，要么使用高盐浓度来诱导复合物解离。我们的结果表明，典型的系统性红斑狼疮血清中至少含有两种不同类型的抗双链DNA抗体。一类抗体亲和力相当高，在存在过量DNA或高盐的情况下解离缓慢。另一类抗体亲和力则低得多，在这些条件下解离更快。双标记解离动力学研究的结果提供了支持这一假设的独立证据。
