Small R C, Weston A H
J Pharmacol Methods. 1980 Jan;3(1):33-8. doi: 10.1016/0160-5402(80)90062-5.
The construction and use of tissue holders that allow simultaneous long-term recordings of mechanical and intracellular electrical activity from a variety of smooth-muscle preparations is described. The tissue holders permit adjustment of tissue stretch following its fixation for recording purposes. By this means intracellular microelectrodes can be kept in position despite marked changes in the mechanical activity of the tissue. Since simultaneous recordings of electrical and mechanical activity of up to 2 hrs duration can be made, studies of the relationships between the membrane potential changes and mechanical changes evoked by drugs and neurotransmitters are greatly facilitated.
本文描述了一种组织固定器的构建和使用方法，该固定器能够同时对多种平滑肌制剂进行长期的机械活动和细胞内电活动记录。这种组织固定器允许在固定组织以进行记录后对组织拉伸进行调整。通过这种方式，尽管组织的机械活动发生了显著变化，细胞内微电极仍可保持在原位。由于能够同时进行长达2小时的电活动和机械活动记录，因此极大地促进了对药物和神经递质引起的膜电位变化与机械变化之间关系的研究。
